Friday, April 18, 2014

Ten Lessons from Biotechnology Experiences in Developing Countries

The Asian Biotechnology and Development Review has published an article, "Ten Lessons from Biotechnology Experiences in Crops, Livestock, and Fish for Smallholders in Developing Countries." Written by James D. Dargie, John Ruane, and Andrea Sonnino, the article is a project by the Food and Agriculture Organization of the United Nations (FAO). 
The FAO commissioned a unique series of 19 case studies where agricultural biotechnologies were used to serve the needs of smallholders in developing countries. Most involved a single crop, livestock or fish species and a single biotechnology. From the case studies, ten general and interrelated lessons were drawn that could be used to inform and assist policymakers when deciding on potential interventions involving biotechnologies for smallholders.
Some of the lessons are:
  • the need for government commitment and backing from donors and international agencies;
  • the need for partnerships, both nationally and internationally, but also with the farmers themselves; and,
  • the recognition that long-term investments in science and technology are critical, as is the appropriate integration of biotechnologies with science-based and traditional knowledge.
The study also found that planning, monitoring, and evaluation of biotechnology applications was weak and should be strengthened.
The article is available for download at: http://www.fao.org/docrep/019/as351e/as351e.pdf.

ISAAA Brief 46-2013: Top Ten Facts

ISAAA releases Top Ten Facts about Biotech/GM Crops in 2013, a Special Edition of Crop Biotech Update March 5 issue. We encourage you to translate and/or use the information therein to develop news articles for publication in tri-media in your own country, with proper attribution to ISAAA.

FACT # 1. 2013 was the 18th year of successful commercialization of biotech crops.

Biotech crops were first commercialized in 1996. Hectarage of biotech crops increased every single year between 1996 to 2013, with 12 years of double-digit growth rates, reflecting the confidence and trust of millions of risk-averse farmers around the world, in both developing and industrial countries. Remarkably, since the first plantings in 1996, an unprecedented cumulative hectarage of more than1.5 billion hectares have been successfully cultivated, an area that is 50% more than the total land mass of China or the United States.

FACT # 2. Biotech crop hectares increased by more than 100-fold from 1.7 million hectares in 1996, to over 175 million hectares in 2013.

This makes biotech crops the fastest adopted crop technology in recent times – the reason – they deliver benefits. In 2013, hectarage of biotech crops grew by 5 million hectares, at an annual growth rate of 3%. It is important to note that more modest annual gains, and continued plateauing, are predicted for the next few years due to the already optimal (between 90% and 100%) adoption rates for the principal biotech crops, leaving little or no room for expansion.

FACT # 3. Number of countries growing biotech crops and stacked traits.

Of the 27 countries which planted biotech crops in 2013, 19 were developing and 8 were industrial countries. Stacked traits occupied 47.1 million hectares, or 27%.

FACT # 4. For the second consecutive year, in 2013, developing countries planted more hectares than industrial countries.

Notably, developing countries grew more, 54% (94 million hectares) of global biotech crops in 2013 than industrial countries at 46% (81 million hectares). Successful public/private partnerships were established by several countries including Brazil, Bangladesh and Indonesia.

FACT # 5. Number of farmers growing biotech crops.

In 2013, a record 18 million farmers, up 0.7 million from 2012, grew biotech crops – remarkably over 90%, or over 16.5 million, were small resource-poor farmers in developing countries. Farmers are the masters of risk-aversion and improve productivity through sustainable intensification (confining cultivation to the 1.5 billion hectares of cropland and thereby saving the forests and biodiversity). In 2013, a record 7.5 million small farmers in China and another 7.3 million in India, elected to plant more than 15 million hectares of Bt cotton, because of the significant benefits it offers. In 2013, almost 400,000 small farmers in the Philippines benefited from biotech maize.

FACT # 6. The top 5 countries planting biotech crops – deployment of the first drought tolerant maize and stacked HT/IR soybean.

The US continued to be the lead country with 70.1 million hectares, with an average ~90% adoption across all crops. Importantly, the first biotech drought tolerant maize was planted by 2,000 US farmers on 50,000 hectares. Brazil was ranked second, and for the fifth consecutive year, was the engine of growth globally, increasing its hectarage of biotech crops more than any other country – an impressive record increase of 3.7 million hectares, up 10% from 2012, reaching 40.3 million hectares. Brazil also planted the first stacked HT/IR soybean in a record-breaking 2.2 million hectare launch, and its home-grown virus-resistant biotech bean is ready for commercialization. Argentina retained its third place with 24.4 million hectares. India, which displaced Canada for the fourth place had a record 11 million hectares of Bt cotton with an adoption rate of 95%. Canada was fifth at 10.8 million hectares with decreased plantings of canola but maintained a high adoption rate of 96%. In 2013, each of the top 5 countries planted more than 10 million hectares providing a broad, solid foundation for future growth.

FACT # 7. Status of biotech crops in Africa.

The continent continued to make progress with South Africa benefiting from biotech crops for more than a decade. Both Burkina Faso and Sudan increased their Bt cotton hectarage by an impressive 50% and 300%, respectively, in 2013. Seven countries (Cameroon, Egypt, Ghana, Kenya, Malawi, Nigeria and Uganda) conducted field trials, the penultimate step prior to approval for commercialization. Importantly, the WEMA project is scheduled to deliver the first biotech drought tolerant maize to Africa in 2017. The lack of appropriate, science-based and cost/time-effective regulatory systems continues to be the major constraint to adoption. Responsible, rigorous but not onerous, regulation is needed, particularly for small and poor developing countries.

FACT # 8. Status of biotech crops in the EU.

Five EU countries planted a record 148,013 hectares of biotech Bt maize, up 15% from 2012. Spain led the EU with 136,962 hectares of Bt maize, up 18% from 2012 with a record 31% adoption rate in 2013.

FACT # 9. Benefits offered by biotech crops.

From 1996 to 2012, biotech crops contributed to Food Security, Sustainability and the Environment/Climate Change by: increasing crop production valued at US$116.9 billion; providing a better environment, by saving 497 million kg a.i. of pesticides; in 2012 alone reducing CO2 emissions by 26.7 billion kg, equivalent to taking 11.8 million cars off the road for one year; conserving biodiversity by saving 123 million hectares of land from 1996-2012; and helped alleviate poverty for >16.5 million small farmers and their families totalling >65 million people, who are some of the poorest people in the world. Biotech crops are essential but are not a panacea and adherence to good farming practices such as rotations and resistance management, are a must for biotech crops as they are for conventional crops.

FACT # 10. Future Prospects.

Cautiously optimistic with more modest annual gains expected due to the already high rates of adoption (90% or more) in the principal biotech crops in mature markets in both developing and industrial countries. Bangladesh, Indonesia and Panama approved biotech crop planting in 2013 with plans for commercialization in 2014.

Peanut Gets an Upgrade Against Drought and Salinity

Peanut (Arachis hypogaea L.) is one of the economically important oil and food crops. Peanut is generally grown across a wide range of environments including rain-fed conditions. Because of this, drought is a main limiting factor to peanut production in the semi-arid areas. The development of salinity and drought stress-tolerant peanut to exploit the drought-prone and salinity-affected areas of the world has been imperative these past years. Now, mannitol may just be the thing that would make it a reality.
Mannitol accumulation in most plants works for the alleviation of salinity and osmotic-induced stresses. However, it is not naturally synthesized in peanut. The mtlD gene (from Escherichia coli) codes for an enzyme that converts fructose 2 with 6-phosphate to mannitol1-phosphate. Peanuts transformed with mtlD were evaluated for salinity and drought stress tolerance. The overexpression of the mtlD gene translated to the transgenic peanuts' improved tolerance to salinity and drought. This was revealed by better growth and physiological parameters like mannitol content, total chlorophyll content, and relative water content in transgenic peanuts.
The better performance of the transgenic plants was attributed to the stress-shielding role of mannitol. However, the mtlD expression causing the activation of other protective reactions in transgenic peanut may also be possible

 Read more at: http://www.cropj.com/thankappan_8_3_2014_413_421.pdf.

Punctured-hypocotyl Method of Agrobacterium-mediated Transformation

Tomato productivity has always been constrained because of abiotic stresses. Transgenic tomatoes are presently being developed to minimize these losses due to abiotic stresses. Agrobacterium-mediated transformation is the most common approach to producing transgenic tomato. However, the effectiveness of the present methods were limited to only a few tomato cultivars. Hence, we still need an appropriate, simple and general procedure effective across all cultivars. Wounding methods, such as puncturing with a syringe needle, may just be the answer.
Using Indian tomato hypocotyl explants, the efficiencies of the punctured-hypocotyl method as well as normal immersion method of Agrobacterium-mediated transformation were compared. All factors influencing transformation efficiency, such as Agrobacterium density and co-cultivation time, were optimized. The transgene integration of the tomato genome was confirmed by PCR and Southern hybridization. Transformation efficiency was found to be greater with the punctured-hypocotyl method compared to the normal immersion method.
This newly developed method is simple, efficient and could be used to transfer important agronomic genes into the tomato genome for the potential improvement in terms of quality and quantity.
read more on http://www.sciencedirect.com/science/article/pii/S0304423813006237

Monday, March 17, 2014

Tanzania biotech debate heats up

DAR ES SALAAM, Tanzania – The government has been advised to play its part by creating conducive environment so as to enable local researchers to do their job on the merit and shortcoming of biotech crops.
The adoption of biotech crops has been a contentious debate in Tanzania. Scientists say the technology has no health effects as it has been propagated by activists while policy makers are hesitant to make a decision either way.
The ongoing debate has caused panic among many farmers who due to bad weather have suffered poor harvests.
According to a study by the European Academies Science Advisory Council (EASAC), titled ‘Planting the future: opportunities and challenges for using crop genetic improvement technologies for sustainable agriculture,’ a billion people on this planet experience hunger.
The EASAC study goes on to state that another billion eat a diet lacking in essential vitamins and minerals.
According to the study, as the debate on GM technology continues, the world’s population continues to grow and, over the next 40 years, agricultural production will have to increase by some 60%.
But several Ugandan and Tanzanian scientists say the future is bright because these challenges can beaten by GM technology.
“What is missing is the government willingness to allow the widely use of biotech crops,” Chief Researcher, from the Tanzania Commission for Science and Technology (COSTECH), Dr Nicholaus Nyange said.
Dr Nyange said, biotech crops could be used as an alternative to solve the said challenges that have been facing Africa farmers most of the time. The issue of climate change, drought and disease.
In Uganda, Head of the Biotechnology Centre at Kawanda, Dr. Andrew Kiggundu said they have developed new varieties of bananas resistant to the devastating banana bacterial wilt disease, nematodes and weevils.
Dr Kiggundu told a group of Ugandan and Tanzania journalists, who toured the centre as part of their field trip to learn about biotech organized by the Bioscience for Farming in Africa, that the new varieties developed in collaboration with the Queensland University of Technology, Australia, are still being monitored in confined field trial gardens at the research institute.
The Kawanda’s researchers have also fortified yellow bananas (Ndiizi), mostly eaten as fruits, with Vitamin A, Zinc and Iron.
The three nutrients, essential for proper growth in children, intellectual development and supply of blood in the body, were got from genes of maize and a special type of foreign bananas called Aspina.
“Banana is a staple food. Some people can eat bananas daily but still lack these nutrients. A number of children are stunted while many expectant mothers die due to lack of enough blood. This is what the new varieties are to address,” Kiggundu said.
Although genetic modification has attracted the closest attention, it is only one of a clutch of new breeding technologies to have been developed in recent decades. The term GM is generally taken to mean the introduction into an organism of genetic material from a different species.
The Managing Director of Tanseed International, Isaka Mashauri said last week recently the government has to allow farmers to choose the seeds they want, whether traditional or biotech crops.
According to Mashauri, who his firm engaged in quality seed production and marketing of crop varieties, the ongoing debate about GM technology is confusing farmers. “Farmers are in dilemma now,” Mashauri said.
“What the government has to do, is to create  a conducive environment for researchers to do their job, so that farmers will be able to know the bad and good of biotech crops,”
The scientists are the only the communities that could clearly articulate the consequences of research findings and the opportunities for agricultural innovation,” Dr Nyange said.
He said the regulatory framework for crop genetic improvement technologies must be reformulated appropriately to be science-based, transparent, proportionate and predictable, taking into account the extensive experience gained and good practice implemented worldwide.
According to Mashauri, if the government will be able to explain the pros and cons of using biotech crops will help farmers decide on whether to adopt the technology or not.
He said that people are hesitant to use biotech crops because they do not know their impacts on their lives, urging the government to impart more knowledge on the organisms.
The global value of biotech seed alone was $13.2 billion in 2011, with the end product of commercial grain from biotech maize, soybean grain and cotton valued at approximately $160 billion or more per year.
Players in agriculture business markets include seed companies, agrochemical companies, distributors, farmers, grain elevators, and universities that develop new crops and whose agricultural extensions advise farmers on best practices
source: East african business week website

Tuesday, March 11, 2014

Medicinal Power of Human Urine!

The title of the article itself must have caught a good deal of your attention! Most of you probably might have been getting a yuck! feeling imagining the consumption of Human Urine as a Medicine! But it's true! Human Urine is more than just an excretory-waste liquid thrown out of the body a couple of times a day.
Rather, there are companies making Billions of dollars by selling drugs extracted from Human Urine, and there are instances in the world where people have been cured of diseases like chronic cystitis drinking their own Urine! So, the focus of this article is to deliver insights into the concept of Human Urine as a Medicine.

"Urine is Sterile Liquid!"

Firstly, one should get rid of the notion that Urine might be containing a lot of contamination/microbes. Human Urine is aseptic and ultra-sterile. Only those humans, suffering from some UTI (Urinary Tract Infection) tend to excrete microbe infected urine. Otherwise, Human Urine is free of infectious organisms. So, this removes one of the key hurdles towards use of urine as a medicine.

Human Urine is Free of Toxic Metabolites
Another important fact one should be aware of is that " Human Urine is Free of Toxic Metabolites" It's the job of Human Liver to detoxify the blood (not of kidneys) of toxic metabolites generated upon digestion of food/medicinal intake. The toxins are then excreted out of the body as fecal matter (not in urine). Urine is rather produced in the kidneys, where the job of nephrons (cells, functional unit of kidneys), is to mediate the filtration of blood to rid it off excess urea, salts, Zinc, Iron, Calcium, Manganese, Magnesium, Some Pigments, vitamins, Hormones, Amino Acids,Glucose, and water, to maintain Homeostasis (a condition of balance) in the body.

An Important Point About Urine
It's just a liquid secreted by body to expel excess "important substances" out of the body. Almost every component excreted in urine has a role to play in human body, but it's excreted out only because body has a particular threshold requirement of every element, any amount in excess is needless, and is excreted if that cannot be stored (Best example being vitamins and hormones, which are never stored in body. If they are in excess, they will be coming out of the body in Urine.

Medicinal Components in Urine
Needless to say, almost every component in human urine has a potential to be a medicine (depending upon the cases/deficits). Here are a few categories:

A. Enzymes
Pharma companies are always hankering after the ways to extract enymes/proteins out of the human urine. They are one of the billion dollars components of the urine. Best example to cite this is " Urokinase" an enzyme extracted from urine which is a miracle drug for dissolving blood clots in coronary arteries.

B. Urea
One of the key components excreted out of body in urine. Owing to it's moisturizing capabilities, it's a key constituent of world renowned creams/lotions. Most of you must have used or known someone having used MURINE EAR & EYE DROPS? Well, they are used to moisturize the eyes/ears (rest goes needless to say I guess).

C. Hormones
Another component(s) of Urine with a billion dollar market cap! All the excess hormones like testosterone, FSH, LH etc are secreted in urine. And, each of these is a routine part of Hormone therapy of billions of people round the globe! A popular fertility drug obtained from urine, named "Pergonal" which is a mixture of follicle-stimulating hormones (FSH) and Luteinizing hormone (LH) had a starting market of $800 million in 1992 only. One can easily imagine the size of market it might have covered till date!

D. Vitamins and Amino Acids
Alanine, Arginine, Ascorbic acid, Biotin, Folic acid, Glutamic acid, Glycine, Inositol,Lysine, Methionine, Nitrogen, Pantothenic acid, Phenylalaline, Riboflavin, Tryptophan, Tyrosine, Vitamin B6, Vitamin B12 are some of the common excretory products of urine, but all of them indispensable for the body.
The reason behind Bear Grylls' (Man Vs Wild, Discovery Channel) recommendation for taking your own urine when there's nothing else to survive on, is very valid. Your urine has a lot to contribute to your body in extreme situations of drought/food shortage!

E. Tendency to Free an Allergic Person from Allergies
Research has proved that due to the fact that urine has the antigen receptors responsible for causing allergic responses in a person suffering from allergy to a particular antigen, if the person is made to drink his/her own urine, he/she may develop antibodies against the antigen that can rid him/her off future inflammations! (An immunity to allergy!)

There's a lot one can explore about the medicinal properties of human urine (in India, people are rather obsessed for Cow's Urine too), this was just an attempt to bring a fact into picture.

Antibiotic-resistance genes in viruses in fossilised 14th century human faecal sample

A discovery of viruses harbouring antibiotic-resistance genes in fossilised faecal samples dating from the 14th century could have implications not only for microbiologists but also for archaeologists, historians and anthropologists. The discovery was made by French researchers who studied samples from latrines from the period uncovered during an urban renewal project in the city of Namur in Belgium. The study is published ahead of print in the journal Applied and Environmental Microbiology.

The viruses found in the coprolites (fossilised faecal samples) are phages, i.e. viruses that infect bacteria rather than eukaryotes. These phages were examined by a mixture of “electron microscopy, high-throughput sequencing and suicide PCR approaches” according to corresponding author Christelle Desnues of Aix Marseille Université. The presence of the phages in the coprolites indicates that they would also have been present in the gastrointestinal tract. Many of the phage sequences identified were related to phages known in modern times to infect bacteria commonly identified in stools, including both harmless, helpful and pathogenic bacteria.

The findings revealed that the phages carried genes for antibiotic resistance, long before antibiotics were used therapeutically. The phages also carried toxin-resistance genes. Both antibiotics and toxins are commonly found in nature. The authors believe that these resistance genes would have protected gut bacteria. In this regard, they would have been essential in maintaining gut metabolism and health as the helpful bacteria inhabiting the gut and other body areas are important in human health. The results are consistent with other studies, for example of the human oral microbiome in skeletons of 1000 years old in which antibiotic-resistance genes were also found.

The phages in the coprolites differed taxonomically those within modern human faecal samples. However, Dr Desnues says that functionally their role has conserved. This adds weight to the hypothesis that the viral community in the human gastrointestinal tract play a fundamental role which has been conserved over centuries, despite dramatic changes in human diet and living conditions. The researchers are currently expanding their studies to fungi and parasites in the coprolites.

Sources:

Press release from American Society for Microbiology; available at http://www.eurekalert.org/pub_releases/2...022714.php [Accessed 28 February 2014].

APPELT, S., FANCELLO, L., LE BAILLY, M., RAOULT, D., DRANCOURT, M. and DESNUES, C., 2014. Viruses in a 14th-century coprolite. Appl. Environ. Microbiol. published ahead of print 7 February 2014 doi:10.1128/AEM.03242-13

WARINNER, C., RODRIGUES, J.F.M., VYAS, C., TRACHSEL, R., SHVED, N., GROSSMANN, J., RADINI, A., HANCOCK, Y., TITO, R.Y., FIDDYMENT, S., SPELLER, M., HENDY, J., CHARLTON, S., LUDER, H.U., SALAZAR-GARCÍA, D.C., EPPLER, E., SEILER, R., HANSEN, L.H., SAMANIEGO CASTRUITA, J.A., BARKOW-OESTERREICHER, S., TEOH, K.Y., KELSTRUP, C.D., OLSEN, J.V., NANNI, P., KAWAI, T., WILLERSLEV, E., VON MERING, C., LEWIS JR, C.M., COLLINS, M.J., GILBERT, M.T.P., RÜHLI, F. and CAPPELLINI, E., 2014. Pathogens and host immunity in the ancient human oral cavity. Nature Genetics 2014; doi:10.1038/ng.2906 (Advance online publication).